Anti-angiogenic activity of julibroside J8, a natural product isolated from Albizia julibrissin

Phytomedicine. 2009 Aug;16(8):703-11. doi: 10.1016/j.phymed.2009.01.002. Epub 2009 May 6.

Abstract

Purpose: The purpose of this study was to investigate the anti-angiogenic properties of julibroside J(8), a triterpenoid saponin isolated from Albizia julibrissin.

Methods: In the presence of juliborside J(8,) the growth of human microvascular endothelial cells (HMEC-1), four human tumor cell lines, and a normal cell line (MRC-5) was evaluated by MTT assay. The in vivo anti-angiogenic effect of julibroside J(8) was evaluated on a chorioallantoic membrane (CAM) and in transplanted colon carcinoma cells in a nude mice neovascularisation model.

Results: Treatment with 0.5-4 microg/ml julibroside J(8) resulted in dose-dependent inhibition of growth, migration, and tube formation in HMEC-1 cells; julibroside J(8) also inhibited the formation of microvessels on CAM at a concentration of 10-50 microg/egg and reduced vessel density within tumor at a concentration of 0.5-3mg/kg.

Conclusions: Julibroside J(8) may be a potent anti-angiogenetic and cytotoxic drug; further investigation is warranted.

MeSH terms

  • Albizzia / chemistry*
  • Angiogenesis Inhibitors / chemistry
  • Angiogenesis Inhibitors / pharmacology*
  • Angiogenesis Inhibitors / therapeutic use
  • Animals
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Antineoplastic Agents, Phytogenic / therapeutic use
  • Cell Line
  • Cell Line, Tumor
  • Chick Embryo
  • Chorioallantoic Membrane
  • Colonic Neoplasms
  • Dose-Response Relationship, Drug
  • Endothelial Cells
  • Humans
  • Mice
  • Microvessels / drug effects
  • Phytotherapy
  • Plant Bark
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Plant Stems
  • Plants, Medicinal / chemistry
  • Polysaccharides / chemistry
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Polysaccharides / therapeutic use
  • Saponins / pharmacology*
  • Saponins / therapeutic use

Substances

  • Angiogenesis Inhibitors
  • Antineoplastic Agents, Phytogenic
  • Julibroside J8
  • Plant Extracts
  • Polysaccharides
  • Saponins