Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome

Science. 2009 May 8;324(5928):804-7. doi: 10.1126/science.1168683.

Abstract

Visibly fluorescent proteins (FPs) from jellyfish and corals have revolutionized many areas of molecular and cell biology, but the use of FPs in intact animals, such as mice, has been handicapped by poor penetration of excitation light. We now show that a bacteriophytochrome from Deinococcus radiodurans, incorporating biliverdin as the chromophore, can be engineered into monomeric, infrared-fluorescent proteins (IFPs), with excitation and emission maxima of 684 and 708 nm, respectively; extinction coefficient >90,000 M(-1) cm(-1); and quantum yield of 0.07. IFPs express well in mammalian cells and mice and spontaneously incorporate biliverdin, which is ubiquitous as the initial intermediate in heme catabolism but has negligible fluorescence by itself. Because their wavelengths penetrate tissue well, IFPs are suitable for whole-body imaging. The IFPs developed here provide a scaffold for further engineering.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics
  • Amino Acid Sequence
  • Animals
  • Biliverdine* / chemistry
  • Biliverdine* / metabolism
  • Cell Line
  • Deinococcus / chemistry*
  • Diagnostic Imaging
  • Fluorescence
  • Humans
  • Liver / anatomy & histology
  • Luminescent Proteins* / chemistry
  • Luminescent Proteins* / metabolism
  • Mice
  • Molecular Sequence Data
  • Phytochrome* / chemistry
  • Phytochrome* / genetics
  • Phytochrome* / metabolism
  • Protein Engineering*
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Spectrophotometry, Infrared
  • Whole Body Imaging

Substances

  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Phytochrome
  • Biliverdine