The effects of body temperature control on cytokine production in a rat model of ventilator-induced lung injury

Cytokine. 2009 Jul;47(1):48-55. doi: 10.1016/j.cyto.2009.04.004. Epub 2009 May 8.

Abstract

Background and purpose: Injurious ventilation with high peak inspiratory pressure (PIP) is known to cause systemic inflammatory response through cytokine production. This study was performed to examine whether body temperature could regulate cytokine production in ventilator-induced lung injury (VILI) model.

Methods: After performing anesthesia, tracheostomy, and catheter insertion, rats were ventilated with 17cmH(2)O of PIP in the low-pressure (LP) group or 35cmH(2)O in the high-pressure (HP) group. Then, each group was divided into three subgroups; hyperthermia (39 degrees C), normothermia (37 degrees C), and hypothermia (34 degrees C) group. Six groups were observed for 6h.

Results: Plasma levels of pro-inflammatory cytokines, TNF-a and IL-6 at 1h after the start of observation were highest in 39 degrees C-HP group and were lowest in 34 degrees C-HP group. Furthermore, sustained high plasma levels of IL-6 were observed only in 39 degrees C-HP group. In contrast, plasma levels of anti-inflammatory cytokine, IL-10 at 1h were highest in 34 degrees C-HP group, and lowest in 39 degrees C-HP group.

Conclusion: The body temperature significantly affects cytokine production in a model of VILI. Body temperature control may be a potentially effective therapeutic modality to regulate cytokine production in VILI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Pressure / physiology
  • Body Temperature / physiology*
  • Bronchoalveolar Lavage Fluid / chemistry
  • Carbon Dioxide / metabolism
  • Chemokine CXCL2 / blood
  • Chemokine CXCL2 / metabolism
  • Cytokines / biosynthesis*
  • Cytokines / blood
  • Disease Models, Animal
  • HMGB1 Protein / blood
  • HMGB1 Protein / metabolism
  • Hydrostatic Pressure
  • Interleukin-10 / blood
  • Interleukin-10 / metabolism
  • Interleukin-1beta / blood
  • Interleukin-1beta / metabolism
  • Interleukin-6 / blood
  • Interleukin-6 / metabolism
  • Lung / metabolism
  • Lung / pathology
  • Male
  • Oxygen / metabolism
  • Partial Pressure
  • Rats
  • Rats, Sprague-Dawley
  • Tumor Necrosis Factor-alpha / blood
  • Tumor Necrosis Factor-alpha / metabolism
  • Ventilator-Induced Lung Injury / metabolism*
  • Ventilator-Induced Lung Injury / pathology

Substances

  • Chemokine CXCL2
  • Cxcl2 protein, rat
  • Cytokines
  • HMGB1 Protein
  • Hbp1 protein, rat
  • Interleukin-1beta
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Carbon Dioxide
  • Oxygen