Huo-Luo-Xiao-Ling Dan modulates antigen-directed immune response in adjuvant-induced inflammation

J Ethnopharmacol. 2009 May 4;123(1):40-4. doi: 10.1016/j.jep.2009.02.032. Epub 2009 Mar 4.


Ethnopharmacological relevance: HLXL is a traditional Chinese medicine that has long been used in folk medicine for the treatment of chronic inflammatory diseases. However, the precise immunological mechanisms by which HLXL mediates its anti-inflammatory activity are not fully defined.

Aim of the study: To determine the effects of HLXL on antigen-specific immune parameters in adjuvant-induced inflammation model in the Lewis rat.

Materials and methods: Rats were fed daily with either HLXL (2.3g/kg) or vehicle (water) beginning 3 days before subcutaneous injection of heat-killed Mycobacterium tuberculosis H37Ra (Mtb), and then continued for another 6 days. After 9 days of Mtb injection, the draining lymph node cells were tested for T cell proliferative and cytokine responses against mycobacterial heat-shock protein 65 (Bhsp65). Moreover, sera were tested for anti-Bhsp65 antibodies and nitric oxide (NO).

Results: HLXL-treated rats showed reduced T cell proliferative response to Bhsp65 compared to control rats. Furthermore, HLXL suppressed IL-17 response but enhanced IL-10 response without much effect on IFN-gamma. HLXL treatment also reduced the levels of anti-Bhsp65 antibodies but not that of NO.

Conclusions: HLXL feeding modulated both the cellular and the humoral immune response to Bhsp65 favoring an anti-inflammatory milieu for the suppression of adjuvant-induced inflammation.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antibody Formation
  • Antigens / immunology*
  • Cytokines / metabolism
  • Disease Models, Animal
  • Inflammation / chemically induced
  • Inflammation / immunology
  • Inflammation / therapy*
  • Inflammation Mediators / metabolism
  • Male
  • Medicine, Chinese Traditional*
  • Rats
  • Rats, Inbred Lew


  • Antigens
  • Cytokines
  • Inflammation Mediators