Anti-inflammatory activity of Chrysanthemum indicum extract in acute and chronic cutaneous inflammation

J Ethnopharmacol. 2009 May 4;123(1):149-54. doi: 10.1016/j.jep.2009.02.009. Epub 2009 Feb 14.


Aim of the study: Although Chrysanthemum indicum Linné (Compositae) has long been used in traditional Korean, Chinese, Japanese medicine to treat various immune-related diseases the underlying mechanism(s) by which these effects are induced remains to be defined in vivo model system. We investigated the effects of 70% ethanolic extract from Chrysanthemum indicum Linné (CIE) on skin inflammation in mice.

Materials and methods: Production of pro-inflammatory cytokines (TNF-alpha and IL-1 beta), activation of myeloperoxidase, and histological assessment were examined in acute and chronic skin inflammation using 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced mouse ear edema.

Results: CIE inhibited topical edema in the mouse ear, following administration at 200mg/kg (i.p.), leading to substantial reductions in skin thickness and tissue weight, inflammatory cytokine production, neutrophil-mediated myeloperoxidase activity, and various histopathological indicators. Furthermore, CIE was effective at reducing inflammatory damage induced by chronic TPA exposure.

Conclusions: These results demonstrate that CIE is an effective anti-inflammatory agent in murine phorbol ester-induced dermatitis, and suggest that the extract may have therapeutic potential in a variety of immune-related cutaneous diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Anti-Inflammatory Agents / therapeutic use
  • Chronic Disease
  • Chrysanthemum / chemistry*
  • Dermatitis / prevention & control*
  • Enzyme Activation
  • Inflammation Mediators / metabolism
  • Interleukin-1beta / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Peroxidase / metabolism
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Tetradecanoylphorbol Acetate / toxicity
  • Tumor Necrosis Factor-alpha / metabolism


  • Anti-Inflammatory Agents
  • Inflammation Mediators
  • Interleukin-1beta
  • Plant Extracts
  • Tumor Necrosis Factor-alpha
  • Peroxidase
  • Tetradecanoylphorbol Acetate