Abstract
Degenerate primers for the [FeFe] hydrogenase (hydA) were developed and used in PCRs to examine hydA in microbial mats that inhabit saltern evaporative ponds in Guerrero Negro (GN), Mexico. A diversity of deduced HydA was discovered that revealed unique variants, which may reflect adaptation to the environmental conditions present in GN.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / genetics*
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DNA Primers / genetics
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Geologic Sediments / microbiology*
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Hydrogenase / genetics*
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Mexico
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Molecular Sequence Data
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Phylogeny
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Polymerase Chain Reaction / methods
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Polymorphism, Genetic*
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Sequence Alignment
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Sequence Analysis, DNA
Substances
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Bacterial Proteins
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DNA Primers
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DNA, Bacterial
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Hydrogenase
Associated data
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GENBANK/FJ623894
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GENBANK/FJ623895
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GENBANK/FJ623896
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GENBANK/FJ623897
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GENBANK/FJ623898
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GENBANK/FJ623899
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GENBANK/FJ623900
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GENBANK/FJ623901
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GENBANK/FJ623902
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GENBANK/FJ623903
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GENBANK/FJ623904
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GENBANK/FJ623905
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GENBANK/FJ623906
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GENBANK/FJ623908
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