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. 2009 Jul;75(13):4627-9.
doi: 10.1128/AEM.00221-09. Epub 2009 May 8.

PirAB toxin from Photorhabdus asymbiotica as a larvicide against dengue vectors

Arunee Ahantarig  1 , Nantarat Chantawat, Nicholas R Waterfield, Richard ffrench-Constant, Pattamaporn Kittayapong
Affiliations

PirAB toxin from Photorhabdus asymbiotica as a larvicide against dengue vectors

Arunee Ahantarig et al. Appl Environ Microbiol. 2009 Jul.

Abstract

We have evaluated Photorhabdus insect-related protein (Pir) from Photorhabdus asymbiotica against dengue vectors. PirAB shows larvicidal activity against both Aedes aegypti and Aedes albopictus larvae but did not affect the Mesocyclops thermocyclopoides predator. PirAB expressed the strongest toxicity compared to PirA, PirB, or the mixture of PirA plus PirB. Whether the presence of an enterobacterial repetitive intergenic consensus sequence in PirAB, but not in PirA, PirB, or the mixture of PirA plus PirB, has any impact on biological control efficacy needs further investigation.

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Figures

FIG. 1.
FIG. 1.
Comparison of the genomic organization of the pirAB operons in two species of Photorhabdus and one strain of Yersinia. PirAB genes are filled in red. Note that the presence of mobile element-related sequences (filled in black) and the G+C content of the regions suggest recent horizontal acquisition of these genes. Also, note that the Yersinia pirAB genes are tightly linked to a type I secreted metalloprotease operon, suggesting a larger pathogenicity island. ERIC sequences in the Photorhabdus operons are indicated by vertical arrows. Dotted horizontal arrows show the PCR-generated clones tested in this work.
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