TOPCONS: consensus prediction of membrane protein topology

Nucleic Acids Res. 2009 Jul;37(Web Server issue):W465-8. doi: 10.1093/nar/gkp363. Epub 2009 May 8.


TOPCONS ( is a web server for consensus prediction of membrane protein topology. The underlying algorithm combines an arbitrary number of topology predictions into one consensus prediction and quantifies the reliability of the prediction based on the level of agreement between the underlying methods, both on the protein level and on the level of individual TM regions. Benchmarking the method shows that overall performance levels match the best available topology prediction methods, and for sequences with high reliability scores, performance is increased by approximately 10 percentage points. The web interface allows for constraining parts of the sequence to a known inside/outside location, and detailed results are displayed both graphically and in text format.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Internet
  • Membrane Proteins / chemistry*
  • Protein Conformation
  • Reproducibility of Results
  • Software*


  • Membrane Proteins