Distribution in skin of ceramide after oral administration to rats

Drug Metab Pharmacokinet. 2009;24(2):180-4. doi: 10.2133/dmpk.24.180.

Abstract

In this study, the absorption, distribution and excretion of ceramide were examined in rats. After a single oral administration of (3)H-ceramide, mean plasma concentration of radioactivity reached maximum at approximately 10.67 hr and decreased with a T(1/2) of 67.12 hr. The mean cumulative excretion of radioactivity in urine and feces accounted for approximately 4.79% and 87.44%, respectively, of the dose. At 96 hr after dosing, 1.67% and 3.67%, respectively, of the dose were still present in the skin and carcass. The radioactivity in the skin at 12 hr was lower than that in plasma and the ratio of skin to plasma concentration was 0.7. However, at 120 hr after dosing, the ratio of skin to plasma concentration increased to 4. A detailed analysis of the distribution of radioactivity in a section of skin showed that radioactivity was located in the dermis and epidermis. At 168 hr, the radioactivity in the epidermis was 8.0% of the radioactivity in skin. The results of the present study clearly demonstrate that some ceramide orally administered is distributed gradually in the dermis after gastrointestinal absorption, followed by transfer from the dermis to the epidermis.

MeSH terms

  • Administration, Oral*
  • Animals
  • Ceramides / pharmacokinetics*
  • Ceramides / urine
  • Feces / chemistry*
  • Male
  • Rats
  • Skin / metabolism*
  • Time Factors
  • Tissue Distribution
  • Tritium / chemistry
  • Tritium / pharmacokinetics*

Substances

  • Ceramides
  • Tritium