Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 5 (6), 400-2

Cytosine-5-methyltransferases Add Aldehydes to DNA


Cytosine-5-methyltransferases Add Aldehydes to DNA

Zita Liutkeviciute et al. Nat Chem Biol.


Targeted methylation of cytosine residues by S-adenosylmethionine-dependent DNA methyltransferases modulates gene expression in vertebrates. Here we show that cytosine-5-methyltransferases catalyze reversible covalent addition of exogenous aliphatic aldehydes to their target residues in DNA, thus yielding corresponding 5-hydroxyalkylcytosines. Such atypical enzymatic reactions with non-cofactor-like substrates open new ways for sequence-specific derivatization of DNA and demonstrate enzymatic exchange of 5-hydroxymethyl groups on cytosine in support of an oxidative mechanism of DNA demethylation.

Similar articles

See all similar articles

Cited by 48 articles

See all "Cited by" articles


    1. Hum Mol Genet. 2005 Apr 15;14 Spec No 1:R47-58 - PubMed
    1. Nucleic Acids Res. 2004 Apr 30;32(8):2453-63 - PubMed
    1. J Biol Chem. 1987 Apr 5;262(10):4778-86 - PubMed
    1. Regul Toxicol Pharmacol. 2004 Oct;40(2):92-106 - PubMed
    1. Nucleic Acids Res. 2008 Jun;36(10):e57 - PubMed

Publication types

LinkOut - more resources