SLC2A2 gene expression in kidney of diabetic rats is regulated by HNF-1alpha and HNF-3beta

Mol Cell Endocrinol. 2009 Jun 16;305(1-2):63-70. doi: 10.1016/j.mce.2009.02.014. Epub 2009 Mar 4.


We hypothesize that, in kidney of diabetic rats, hepatocyte nuclear factors (HNF-1alpha and HNF-3beta) play a critical role in the overexpression of solute carrier 2A2 (SLC2A2) gene. Diabetic rats submitted or not to rapid (up to 12h) and short-term (1, 4 and 6 days) insulin treatment were investigated. Twofold increase in GLUT2 mRNA was observed in diabetic, accompanied by significant increases in HNF-1alpha and HNF-3beta expression and binding activity. Additional 2-fold increase in GLUT2 mRNA and HNF-3beta expression/activity was observed in 12-h insulin-treated rats. Six-day insulin treatment decreased GLUT2 mRNA and HNF-1alpha expression and activity to levels of non-diabetic rats, whereas HNF-3beta decreased to levels of non-insulin-treated diabetic rats. Our results provide evidence for a link between the overexpression of SLC2A2 gene and the transcriptional activity of HNF-1alpha and HNF-3beta in kidney of diabetic rats. Furthermore, recovery of SLC2A2 gene after 6-day insulin treatment also involves HNF-1alpha and HNF-3beta activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Diabetes Mellitus / genetics
  • Diabetes Mellitus / metabolism
  • Electrophoretic Mobility Shift Assay
  • Gene Expression Regulation / drug effects
  • Glucose Transporter Type 2 / genetics
  • Glucose Transporter Type 2 / metabolism
  • Glucose Transporter Type 2 / physiology*
  • Hepatocyte Nuclear Factor 1-alpha / physiology*
  • Hepatocyte Nuclear Factor 3-beta / physiology*
  • Insulin / pharmacology
  • Kidney / drug effects
  • Kidney / metabolism*
  • Male
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar


  • Glucose Transporter Type 2
  • Hepatocyte Nuclear Factor 1-alpha
  • Insulin
  • RNA, Messenger
  • Slc2a2 protein, rat
  • Hepatocyte Nuclear Factor 3-beta