Abstract
A 30 second burst of microwave irradiation at an energy level insufficient to cause DNA denaturation or damage drives the covalent reaction between pyrrolobenzodiazepine (PBD) antitumour agents and double-stranded or hairpin oligonucleotides to completion, a process that normally takes between 3-24 hours and thus offering the opportunity for higher-throughput screening of covalent-binding DNA-interactive agents.
MeSH terms
-
Antineoplastic Agents / chemistry
-
Antineoplastic Agents / metabolism*
-
Antineoplastic Agents / radiation effects
-
Benzodiazepines / chemistry
-
Benzodiazepines / metabolism*
-
Benzodiazepines / radiation effects
-
Binding Sites
-
Chromatography, High Pressure Liquid
-
DNA / chemistry
-
DNA / metabolism*
-
DNA / radiation effects*
-
Ligands
-
Microwaves*
-
Models, Molecular
-
Pyrroles / chemistry
-
Pyrroles / metabolism*
-
Pyrroles / radiation effects
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
-
Time Factors
Substances
-
Antineoplastic Agents
-
Ligands
-
Pyrroles
-
pyrrolo(2,1-c)(1,4)benzodiazepine
-
Benzodiazepines
-
DNA