A role for the JAK-STAT1 pathway in blocking replication of HSV-1 in dendritic cells and macrophages

Virol J. 2009 May 13;6:56. doi: 10.1186/1743-422X-6-56.

Abstract

Background: Macrophages and dendritic cells (DCs) play key roles in host defense against HSV-1 infection. Although macrophages and DCs can be infected by herpes simplex virus type 1 (HSV-1), both cell types are resistant to HSV-1 replication. The aim of our study was to determine factor (s) that are involved in the resistance of DCs and macrophages to productive HSV-1 infection.

Results: We report here that, in contrast to bone marrow-derived DCs and macrophages from wild type mice, DCs and macrophages isolated from signal transducers and activators of transcription-1 deficient (STAT1-/-) mice were susceptible to HSV-1 replication and the production of viral mRNAs and DNA. There were differences in expression of immediate early, early, and late gene transcripts between STAT1+/+ and STAT1-/- infected APCs.

Conclusion: These results suggest for the first time that the JAK-STAT1 pathway is involved in blocking replication of HSV-1 in DCs and macrophages.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cells, Cultured
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism
  • Dendritic Cells / virology*
  • Female
  • Gene Expression
  • Herpes Simplex / immunology
  • Herpes Simplex / metabolism*
  • Herpes Simplex / virology
  • Herpesvirus 1, Human / physiology*
  • Humans
  • Janus Kinases / genetics
  • Janus Kinases / metabolism*
  • Macrophages / immunology
  • Macrophages / metabolism
  • Macrophages / virology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • STAT1 Transcription Factor / genetics
  • STAT1 Transcription Factor / metabolism*
  • Signal Transduction*
  • Virus Replication*

Substances

  • STAT1 Transcription Factor
  • Stat1 protein, mouse
  • Janus Kinases