Adoptive transfer of mFas ligand into dendritic cells influences the spontaneous resorption rate in the CBA/J x DBA/2 mouse model

Fertil Steril. 2010 Mar 15;93(5):1700-5. doi: 10.1016/j.fertnstert.2009.01.144. Epub 2009 May 12.

Abstract

Objective: To investigate the effect of mFasL dendritic cells (DC) on the embryo resorption rate in the CBA/J x DBA/2 abortion mouse model.

Design: Experimental study.

Setting: University hospital in a major city in China.

Animal(s): 101 CBA/J mice and 50 DBA/2 mice.

Intervention(s): We constructed the eukaryotic expression vector pcDNA3.1-mFasL, derived the DCs from bone marrow of DBA/2 mice, and transfected the DCs with pcDNA3.1-mFasL. The abortion mouse model were established by mating female CBA/J mice with DBA/2 mice. Via the CBA/J x DBA/2 abortion mouse model, five groups were established: group 1: abortion model without treatment; group 2: abortion mouse model injected with DC culture medium (DCCM); group 3: abortion mouse model injected with DC; group 4: abortion mouse model injected with empty plasmid pcDNA3.1-DC; group 5: abortion model mouse injected with pcDNA3.1-FasL-DC.

Main outcome measure(s): Comparison of difference in the embryo resorption rates of the CBA/J x DBA/2 abortion mouse model treated with either pcDNA3.1-mFasL-DC or different controls observed on gestation days 12 to 14.

Result(s): The embryo resorption rate was statistically significantly decreased in group 3 treated with DC and group 4 with empty plasmid pcDNA3.1-DC when they compared with group 1 (no treatment for abortion mouse model) and group 2 (injected with DC culture medium, DCCM). Furthermore, abortion model group 5 (injected with pcDNA3.1-mFasL-DC) showed a statistically significantly decreased in embryo resorption rate when compared with the other four groups, including groups 1 and 2 and groups 3 and group 4.

Conclusion(s): Adoptive transfer of mFasL-DC can statistically significantly reduce the embryo resorption rate in the CBA/J x DBA/2 abortion mouse model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abortion, Habitual / genetics
  • Abortion, Habitual / immunology
  • Abortion, Habitual / therapy*
  • Adoptive Transfer*
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Crosses, Genetic
  • Dendritic Cells / immunology*
  • Disease Models, Animal
  • Embryo Loss / genetics
  • Embryo Loss / immunology
  • Embryo Loss / prevention & control*
  • Fas Ligand Protein / biosynthesis*
  • Fas Ligand Protein / genetics
  • Female
  • Genetic Therapy / methods*
  • Immune Tolerance
  • Mice
  • Mice, Inbred CBA
  • Mice, Inbred DBA
  • Molecular Sequence Data
  • Pregnancy
  • Transfection

Substances

  • Fas Ligand Protein