Microarray analysis of microRNA expression in renal clear cell carcinoma

Eur J Surg Oncol. 2009 Oct;35(10):1119-23. doi: 10.1016/j.ejso.2009.04.010. Epub 2009 May 13.


Aim: To explore the microRNA (miRNA) expression in renal clear cell carcinoma (RCCC).

Methods: We compared the miRNA expression profiles in 11 pairs of RCCC and adjacent nontumorous tissue (NT) from 11 RCCC patients, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences. To verify microarray results, Northern blotting was carried out on 5 randomly selected miRNAs.

Results: Totally 81 miRNAs were identified valid expression in RCCC samples, 48 of which specifically detected in RCCC samples, 17 of which detected downregulated in RCCC compared to NT sample, 2 upregulated and 14 without significant difference. MiRNAs in RCCC tissues exhibit an overall higher expression level than NT tissue. The chip results were confirmed by northern blot analysis.

Conclusion: Our study may help to clarify the molecular mechanisms involved in the pathogenesis of RCCC, and miRNAs potentially serve as a novel diagnostic biomarker of RCCC.

MeSH terms

  • Adult
  • Biomarkers, Tumor / genetics*
  • Biomarkers, Tumor / metabolism
  • Carcinoma, Renal Cell / genetics*
  • Carcinoma, Renal Cell / physiopathology
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Kidney Neoplasms / genetics*
  • Kidney Neoplasms / physiopathology
  • Male
  • MicroRNAs / metabolism*
  • Microarray Analysis
  • Middle Aged


  • Biomarkers, Tumor
  • MIRN145 microRNA, human
  • MIRN17 microRNA, human
  • MIRN221 microRNA, human
  • MicroRNAs