Objective: Studying the kinetics of conjugated linoleic acid (CLA) bioconversion by Lactobacillus plantarum ZS2058.
Methods: We studied the effects of the substrate concentration, cell mass, reaction pH and temperature on the reaction rate of producing CLA by L. plantarum ZS2058, and established the equations of reaction rate at the initial response stage under different substrate concentrations by double-reciprocal plot and Hanes-Woolf plot method.
Results: A marked substrate inhibition effect existed, and the early reaction rate of producing c9, t11-CLA reached the maximum of 15.99 microg/(mL x h) when linoleic acid concentration was 0.4 mg/mL. The early reaction rate increased with the cell mass increase, and when the cell mass was at 5 x 10(10) cfu/mL, the response reaction rate close to the maximum. The optimum pH and temperature of bioconversion of CLA were 6.5 and 40 degrees C. Michaelis constant was obtained by double-reciprocal plot and Hanes-Woolf plot method, the reaction rate equation followed the classic Michaelis-Mentent equation at the low substrate concentration, while there existed in a marked substrate inhibition effect at the high substrate concentration.
Conclusion: Rough exploring the various factors of CLA bioconversion by L. plantarum ZS2058, we established the reaction rate equations of the initial response stage under different substrate concentrations, and got the optimal reaction conditions. These results will, contribute to the CLA production and its physiological functions research.