One-pot fermentation of pladienolide D by Streptomyces platensis expressing a heterologous cytochrome P450 gene

Kazuhiro Machida; Yasuhide Aritoku; Toshio Tsuchida

doi:10.1016/j.jbiosc.2009.01.023

One-pot fermentation of pladienolide D by Streptomyces platensis expressing a heterologous cytochrome P450 gene

J Biosci Bioeng. 2009 Jun;107(6):596-8. doi: 10.1016/j.jbiosc.2009.01.023.

Authors

Kazuhiro Machida¹, Yasuhide Aritoku, Toshio Tsuchida

Affiliation

¹ Bioresource Laboratories, Mercian Corp., 1808 Nakaizumi, Iwata, Shizuoka 438-0078, Japan. machida-k@mercian.co.jp

PMID: 19447333
DOI: 10.1016/j.jbiosc.2009.01.023

Abstract

Pladienolide D is a 16-hydroxylated derivative of pladienolide B, produced by Streptomyces platensis. To facilitate the production of pladienolide D, the gene encoding a pladienolide B 16-hydroxylase from S. bungoensis was introduced into S. platensis. The recombinant produced pladienolide D at a production level comparable to that of pladienolide B.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / metabolism
Cloning, Molecular
Cytochrome P-450 Enzyme System / metabolism*
Epoxy Compounds / metabolism*
Fermentation
Macrolides / metabolism*
Streptomyces / metabolism*

Substances

Bacterial Proteins
Epoxy Compounds
Macrolides
pladienolide B
pladienolide D
Cytochrome P-450 Enzyme System