Cloning and functional analysis of cis-prenyltransferase from Thermobifida fusca

J Biosci Bioeng. 2009 Jun;107(6):620-2. doi: 10.1016/j.jbiosc.2009.02.006.


cis-Prenyltransferase catalyzes the synthesis of Z,E-mixed prenyl diphosphates by a condensation of isopentenyl diphosphate to an allylic diphosphate. A novel gene encoding a cis-prenyltransferase is cloned from Thermobifida fusca. It showed a unique substrate specificity accepting dimethylallyl diphosphate as a shortest allylic substrate, and synthesizes polyprenyl products up to C(70).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / enzymology*
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / metabolism*
  • Cloning, Molecular
  • Substrate Specificity
  • Transferases / isolation & purification
  • Transferases / metabolism*


  • Bacterial Proteins
  • Transferases
  • cis-prenyl transferase