By the methods of classical statistics and geostatistics, the spatial heterogeneity of surface soil (0-5 cm and 5-10 cm layers) moisture content in dry season in the typical sloping fields and depressions in Mulun National Natural Reserve in Karst area were studied. The results indicated that in study area, the surface soil moisture content in dry season was still higher, and showed a fine semivariogram structure as a whole. The spatial distribution of moisture content in 0-5 cm and 5-10 cm soil layers, both for sloping fields and for depressions, fitted exponential model well. Under the same stand conditions, the moisture content in the two soil layers had the similar spatial structure and distribution pattern; while under different stand conditions, there existed obvious difference in the same soil layer. The spatial pattern of surface soil moisture content in sloping fields was characterized by medium spatial autocorrelation, clear patches with well continuum, relatively slow variation of Moran's I index, while that in depressions was characterized by strong spatial autocorrelation, larger variation of Moran' s I index, and more fragmented patches. Therefore, topography, micro-physiognomy, precipitation, human disturbance, and especially vegetation were the most important factors affecting the spatial pattern of soil moisture content in the Mulun National Natural Reserve, and to preserve primary forest should have favorable effect on the regulation of the spatial heterogeneity of soil moisture content in the Reserve.