Whole-cell patch clamp recordings from rhythmically active motoneurons in the isolated spinal cord of the chick embryo

Neurosci Lett. 1991 Jul 22;128(2):211-6. doi: 10.1016/0304-3940(91)90263-s.


Whole-cell patch clamp recordings were obtained during motor activity from electrically identified motoneurons within the spinal cord of the chick embryo maintained in vitro. Most recordings were performed on E11-E13 motoneurons although it was also possible to record from younger cells (E7-E9). Voltage clamp recordings were used to characterize the synaptic currents expressed in femoro-tibialis (extensor) motoneurons during motor activity. These motoneurons exhibited rhythmic excitatory currents with reversal potentials near 0 mV. This powerful technique enables high resolution recordings from identified motoneurons in situ and allows investigation of the membrane and synaptic mechanisms involved in the development of embryonic motility.

MeSH terms

  • Animals
  • Chick Embryo
  • Electrodes
  • Electrophysiology
  • Membrane Potentials / physiology
  • Motor Neurons / physiology*
  • Muscles / innervation
  • Phrenic Nerve / cytology
  • Phrenic Nerve / physiology
  • Spinal Cord / cytology*
  • Synapses / physiology