Transcriptional variation of regulatory and virulence genes due to different media in Staphylococcus aureus

Microb Pathog. 2009 Aug;47(2):94-100. doi: 10.1016/j.micpath.2009.05.001. Epub 2009 May 18.


The pathogenesis of staphylococcal infections is a multifactorial process that depends on expression of different virulence factors. Expression of these factors is controlled by multiple regulatory systems in conjunction with environmental signals. Most of the genetic studies in Staphylococcus aureus have been performed using different growth media, therefore, we examined the effects of different growth media on transcription of the selective target (e.g., hla, hlb, spa, sspA) and regulatory (e.g., agr, sarA family) genes. The results from this study suggest that different growth media have substantial effect on transcription of various genes being analyzed. Interestingly, when compared with the wild-type, the isogenic sarA mutant showed a media-dependent distinct regulatory effect on expression of the target genes.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Culture Media / chemistry*
  • Culture Media / metabolism
  • Gene Expression Regulation, Bacterial
  • Staphylococcus aureus / chemistry
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / metabolism
  • Transcription, Genetic*
  • Virulence Factors / genetics*
  • Virulence Factors / metabolism


  • Bacterial Proteins
  • Culture Media
  • Virulence Factors