Down-regulation of Wnt signaling during apoptosis of human hepatic stellate cells

Hepatogastroenterology. Jan-Feb 2009;56(89):208-12.

Abstract

Background/aims: Hepatic stellate cell apoptosis may play a key role in inhibition of fibrotic hepatic injury. To understand the gene expression profiles of human hepatic stellate cell apoptosis, the global transcript levels in curcumin-induced apoptotic human telomerase reverse transcriptase hepatic stellate cells were analyzed with microarrays.

Methodology: 19K human oligonucleotide microarrays were performed to obtain the gene expression profiles associated with apoptosis in human hepatic stellate cells. The microarray data were verified by real time quantitative PCR and expression of the components of apoptosis and Wnt signaling was analyzed by Western blot.

Results: The apoptosis pathway components, BAX (BCL2-associated X protein) and FLIP (CASP8 and FADD-like apoptosis regulator), had increased or decreased expression in the microarray data, the findings of which the protein levels were consistent. The Wnt signaling pathway components, AXIN2 and FRA1 (FOS-like antigen 1), showed a decreasing expression in the time course microarrays at the protein level.

Conclusion: Our data show various gene expression profiles during apoptosis of human telomerase reverse transcriptase hepatic stellate cells, especially those involved in the apoptosis and Wnt signaling pathways, and demonstrate that the activation of the Wnt signaling pathway is inhibited in curcumin-induced apoptotic human telomerase reverse transcriptase hepatic stellate cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / genetics*
  • Blotting, Western
  • Cells, Cultured
  • Down-Regulation
  • Gene Expression Profiling
  • Hepatic Stellate Cells / metabolism*
  • Humans
  • Liver / cytology*
  • Liver / metabolism*
  • Oligonucleotide Array Sequence Analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction* / genetics
  • Telomerase / genetics
  • Telomerase / metabolism
  • Up-Regulation
  • Wnt Proteins / genetics*
  • Wnt Proteins / metabolism*

Substances

  • Wnt Proteins
  • TERT protein, human
  • Telomerase