The Akt C-terminal modulator protein is an acyl-CoA thioesterase of the Hotdog-Fold family

Biochemistry. 2009 Jun 23;48(24):5507-9. doi: 10.1021/bi900710w.


Herein, we report on an in vitro kinetic activity analysis that demonstrates that the protein known as the Akt C-terminal modulator protein is a broad-range, high-activity acyl-CoA thioesterase. In vitro tests of possible activity regulation by product inhibition or by Akt1 binding gave negative results. Truncation mutants confined the thioesterase activity to the C-terminal domain, consistent with our threading model. The N-terminal domain of unknown fold and function was found to contribute to solubility.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adaptor Proteins, Signal Transducing / chemistry*
  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / metabolism
  • Kinetics
  • Membrane Proteins / chemistry*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Models, Molecular
  • Palmitoyl-CoA Hydrolase / chemistry*
  • Palmitoyl-CoA Hydrolase / metabolism
  • Protein Conformation
  • Thermodynamics
  • Thiolester Hydrolases


  • Adaptor Proteins, Signal Transducing
  • Membrane Proteins
  • THEM4 protein, human
  • Thiolester Hydrolases
  • Palmitoyl-CoA Hydrolase