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. 2009 Aug;18(4):453-63.
doi: 10.1111/j.1365-2583.2009.00885.x. Epub 2009 Apr 24.

An Initial Linkage Map of the West Nile Virus Vector Culex Tarsalis

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Free PMC article

An Initial Linkage Map of the West Nile Virus Vector Culex Tarsalis

M Venkatesan et al. Insect Mol Biol. .
Free PMC article

Abstract

We have constructed the first genetic linkage map for the North American arboviral vector Culex tarsalis. 120 F(2) offspring from a cross between two colonies were genotyped using 25 microsatellites and six inter-simple sequence repeat (ISSR) markers. We resolved four linkage groups which likely correspond to two full-length chromosomes and two arms of the final chromosome. The longest linkage group contains the sex locus and corresponds to chromosome 3. Recombination rates around the sex locus were dramatically higher in females compared to males. The majority of microsatellite loci share sequence identity with regions of the Culex quinquefasciatus genome, whose assembly should aid in anchoring linkage groups to physical chromosomes. This map will aid in identification of loci involved with variable phenotypes in C. tarsalis including WNV susceptibility.

Figures

Figure 1
Figure 1
ISSR loci used in this cross. A total of 6 bands from 4 primers were mapped. Mapped band positions are indicated by arrows. Genotypes of the F0 male and F0 female are shown along with 4 F2 males and 4 F2 females (1–8). m = male, f = female.
Figure 2
Figure 2
Family-specific and composite linkage maps of Culex tarsalis based on recombination frequencies observed in an F2 cross. Maps were derived from recombination estimates using the Kosambi mapping function. Map distances are listed in cM. Those few markers that were uninformative in family-specific maps (Table 3) were placed by linear interpolation for comparative purposes.
Figure 3
Figure 3
Composite F2 linkage map of Culex tarsalis showing loci mapped by homology to the sequenced C. quinquefasciatus genome (indicated by asterisks). Map distances are listed in cM. Map was constructed using the Kosambi function.
Figure 4
Figure 4
Comparison of sex-specific composite maps of linkage group 1/chromosome 3, highlighting the dramatically increased recombination rate around the sex locus in females. Recombination rates across the remainder of the genome did not vary significantly between sexes.

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