Differential roles of OxyR-controlled antioxidant enzymes alkyl hydroperoxide reductase (AhpCF) and catalase (KatB) in the protection of Pseudomonas aeruginosa against hydrogen peroxide in biofilm vs. planktonic culture

FEMS Microbiol Lett. 2009 Jun;295(2):238-44. doi: 10.1111/j.1574-6968.2009.01605.x. Epub 2009 Apr 27.

Abstract

The role of the Pseudomonas aeruginosa OxyR-controlled antioxidants alkyl hydroperoxide reductase CF (AhpCF) and catalase B (KatB) was evaluated in biofilm vs. planktonic culture upon exposure to hydrogen peroxide. AhpCF was found to be critical for survival of biofilm bacteria while KatB was more important for survival of planktonic free-swimming organisms.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antioxidants / metabolism
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Biofilms* / drug effects
  • Biofilms* / growth & development
  • Catalase / drug effects
  • Catalase / genetics
  • Catalase / metabolism*
  • Culture Media
  • Gene Expression Regulation, Bacterial
  • Hydrogen Peroxide / pharmacology*
  • Peroxiredoxins / drug effects
  • Peroxiredoxins / genetics
  • Peroxiredoxins / metabolism*
  • Plankton* / drug effects
  • Plankton* / enzymology
  • Plankton* / growth & development
  • Pseudomonas aeruginosa / drug effects*
  • Pseudomonas aeruginosa / growth & development
  • Pseudomonas aeruginosa / physiology
  • Trans-Activators / metabolism*

Substances

  • Antioxidants
  • Bacterial Proteins
  • Culture Media
  • OxyR protein, Pseudomonas aeruginosa
  • Trans-Activators
  • Hydrogen Peroxide
  • Peroxiredoxins
  • Catalase