Rapid and high-throughput analysis of N-glycans from ovarian cancer serum using a 96-well plate platform

Anal Biochem. 2009 Aug 15;391(2):151-3. doi: 10.1016/j.ab.2009.05.015. Epub 2009 May 18.


We present a rapid and high-throughput human serum N-glycan preparation technology using 96-well plate-based procedures. The released N-glycans from polyvinylidene fluoride (PVDF) membrane filter plate are subsequently loaded to porous graphitic carbon (PGC) containing a 96-well plate to remove salts and other contaminants without sacrificing accuracy or reproducibility. This robust glycan preparation technology is applied to ovarian cancer diagnosis using 5 microl of patient serum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Female
  • Humans
  • Membranes, Artificial
  • Ovarian Neoplasms / blood
  • Ovarian Neoplasms / chemistry*
  • Polysaccharides / blood*
  • Polysaccharides / isolation & purification
  • Polyvinyls / chemistry
  • Solid Phase Extraction / instrumentation*
  • Solid Phase Extraction / methods
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*


  • Membranes, Artificial
  • Polysaccharides
  • Polyvinyls
  • polyvinylidene fluoride