Abstract
We report here the functional analysis of human Regulator of Ribosome Synthesis 1 (RRS1) protein during mitosis. We demonstrate that RRS1 localizes in the nucleolus during interphase and is distributed at the chromosome periphery during mitosis. RNA interference experiments revealed that RRS1-depleted cells show abnormalities in chromosome alignment and spindle organization, which result in mitotic delay. RRS1 knockdown also perturbs the centromeric localization of Shugoshin 1 and results in premature separation of sister chromatids. Our results suggest that a nucleolar protein RRS1 contributes to chromosome congression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cell Cycle Proteins / metabolism
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Cell Nucleolus / metabolism
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Cell Nucleolus / ultrastructure
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Chromosome Pairing
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Chromosome Segregation
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Chromosomes, Human / metabolism*
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Chromosomes, Human / ultrastructure
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HeLa Cells
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Humans
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Microscopy, Fluorescence
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Mitosis
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Nuclear Proteins / antagonists & inhibitors
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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RNA Interference
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RNA, Small Interfering / genetics
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RNA-Binding Proteins
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Spindle Apparatus / metabolism
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Spindle Apparatus / ultrastructure
Substances
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Cell Cycle Proteins
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Nuclear Proteins
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RNA, Small Interfering
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RNA-Binding Proteins
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RRS1 protein, human
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Recombinant Proteins
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SGO1 protein, human