In Silico Selection of RNA Aptamers

Nucleic Acids Res. 2009 Jul;37(12):e87. doi: 10.1093/nar/gkp408. Epub 2009 May 21.

Abstract

In vitro selection of RNA aptamers that bind to a specific ligand usually begins with a random pool of RNA sequences. We propose a computational approach for designing a starting pool of RNA sequences for the selection of RNA aptamers for specific analyte binding. Our approach consists of three steps: (i) selection of RNA sequences based on their secondary structure, (ii) generating a library of three-dimensional (3D) structures of RNA molecules and (iii) high-throughput virtual screening of this library to select aptamers with binding affinity to a desired small molecule. We developed a set of criteria that allows one to select a sequence with potential binding affinity from a pool of random sequences and developed a protocol for RNA 3D structure prediction. As verification, we tested the performance of in silico selection on a set of six known aptamer-ligand complexes. The structures of the native sequences for the ligands in the testing set were among the top 5% of the selected structures. The proposed approach reduces the RNA sequences search space by four to five orders of magnitude--significantly accelerating the experimental screening and selection of high-affinity aptamers.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Base Pairing
  • Computational Biology / methods*
  • Ligands
  • Models, Molecular
  • Nucleic Acid Conformation
  • RNA / chemistry*
  • Sequence Analysis, RNA
  • Thermodynamics

Substances

  • Aptamers, Nucleotide
  • Ligands
  • RNA