Basophils contribute to T(H)2-IgE responses in vivo via IL-4 production and presentation of peptide-MHC class II complexes to CD4+ T cells

Nat Immunol. 2009 Jul;10(7):706-12. doi: 10.1038/ni.1737. Epub 2009 May 24.

Abstract

Basophils express major histocompatibility complex class II, CD80 and CD86 and produce interleukin 4 (IL-4) in various conditions. Here we show that when incubated with IL-3 and antigen or complexes of antigen and immunoglobulin E (IgE), basophils internalized, processed and presented antigen as complexes of peptide and major histocompatibility complex class II and produced IL-4. Intravenous administration of ovalbumin-pulsed basophils into naive mice 'preferentially' induced the development of naive ovalbumin-specific CD4+ T cells into T helper type 2 (T(H)2) cells. Mice immunized in this way, when challenged by intravenous administration of ovalbumin, promptly produced ovalbumin-specific IgG1 and IgE. Finally, intravenous administration of IgE complexes rapidly induced T(H)2 cells only in the presence of endogenous basophils, which suggests that basophils are potent antigen-presenting cells that 'preferentially' augment T(H)2-IgE responses by capturing IgE complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antigen-Presenting Cells / cytology
  • Antigen-Presenting Cells / immunology*
  • Antigen-Presenting Cells / metabolism
  • Basophils / immunology*
  • Basophils / metabolism
  • Basophils / transplantation
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / metabolism
  • Flow Cytometry
  • Histocompatibility Antigens Class II / chemistry
  • Histocompatibility Antigens Class II / immunology*
  • Immunoglobulin E / immunology*
  • Interleukin-4 / genetics
  • Interleukin-4 / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Ovalbumin / immunology
  • Peptides / chemistry
  • Peptides / immunology
  • Spleen / cytology
  • Spleen / immunology
  • Strongyloides / immunology
  • Strongyloidiasis / immunology
  • Strongyloidiasis / parasitology
  • Th2 Cells / immunology

Substances

  • Histocompatibility Antigens Class II
  • Peptides
  • Interleukin-4
  • Immunoglobulin E
  • Ovalbumin