Remorin, a solanaceae protein resident in membrane rafts and plasmodesmata, impairs potato virus X movement

Plant Cell. 2009 May;21(5):1541-55. doi: 10.1105/tpc.108.064279. Epub 2009 May 26.

Abstract

Remorins (REMs) are proteins of unknown function specific to vascular plants. We have used imaging and biochemical approaches and in situ labeling to demonstrate that REM clusters at plasmodesmata and in approximately 70-nm membrane domains, similar to lipid rafts, in the cytosolic leaflet of the plasma membrane. From a manipulation of REM levels in transgenic tomato (Solanum lycopersicum) plants, we show that Potato virus X (PVX) movement is inversely related to REM accumulation. We show that REM can interact physically with the movement protein TRIPLE GENE BLOCK PROTEIN1 from PVX. Based on the localization of REM and its impact on virus macromolecular trafficking, we discuss the potential for lipid rafts to act as functional components in plasmodesmata and the plasma membrane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / analysis
  • Carrier Proteins / metabolism
  • Carrier Proteins / physiology*
  • Cell Fractionation
  • Green Fluorescent Proteins / analysis
  • Immunity, Innate
  • Membrane Microdomains / metabolism*
  • Molecular Sequence Data
  • Phosphoproteins / analysis
  • Phosphoproteins / metabolism
  • Phosphoproteins / physiology*
  • Plant Diseases / virology
  • Plant Proteins / analysis
  • Plant Proteins / metabolism
  • Plant Proteins / physiology*
  • Plants, Genetically Modified / virology
  • Plasmodesmata / metabolism*
  • Potexvirus / physiology*
  • Recombinant Fusion Proteins / analysis
  • Solanum lycopersicum / metabolism
  • Solanum lycopersicum / ultrastructure
  • Solanum lycopersicum / virology*
  • Virus Replication

Substances

  • Carrier Proteins
  • Phosphoproteins
  • Plant Proteins
  • Recombinant Fusion Proteins
  • remorin
  • Green Fluorescent Proteins

Associated data

  • GENBANK/AAD28506
  • GENBANK/AAD28507
  • GENBANK/BI176676
  • GENBANK/CK269035
  • GENBANK/DN921711
  • GENBANK/EB449751
  • GENBANK/EB451162
  • GENBANK/P93788