Detection of Borrelia Burgdorferi in Biological Samples Using the Polymerase Chain Reaction Assay

Res Microbiol. 1991 Jun;142(5):565-72. doi: 10.1016/0923-2508(91)90189-h.

Abstract

Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the OspB gene. The last set of primers, OspBpc3/pc4, outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids.

MeSH terms

  • Borrelia burgdorferi Group / isolation & purification*
  • Electrophoresis, Agar Gel
  • Humans
  • In Vitro Techniques
  • Lyme Disease / diagnosis*
  • Lyme Disease / microbiology
  • Polymerase Chain Reaction / methods*
  • Synovial Fluid / microbiology