Abstract
Ribosome-inactivating proteins (RIPs) represent a type of protein that universally inactivates the ribosome thus inhibiting protein biosynthesis. Curcin-L was a type I RIP found in Jatropha curcas L.. Its expression could be activated in leaves by treatments with abscisic acid, salicylic acid, polyethylene glycol, temperature 4, 45 degrees C and ultraviolet light. A 654 bp fragment of a 5' flanking region preceding the curcin-L gene, designated CP2, was cloned from the J. curcas genome and its expression pattern was studied via the expression of the beta-glucuronidase (GUS) gene in transgenic tobacco. Analysis of GUS activities showed that the CP2 was leaf specific, and was able to drive the expression of the reporter gene under stress-induction conditions. Analysis of a series of 5'-deletions of the CP2 suggested that several promoter motifs were necessary to respond to environmental stresses.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / pharmacology
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Fluorometry
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Gene Expression Regulation, Plant* / drug effects
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Gene Expression Regulation, Plant* / genetics
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Gene Expression Regulation, Plant* / radiation effects
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Jatropha / drug effects
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Jatropha / genetics*
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Jatropha / radiation effects
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Molecular Sequence Data
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Nicotiana / drug effects
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Nicotiana / genetics*
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Nicotiana / radiation effects
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Plant Leaves / genetics*
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Plant Proteins / genetics
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Plants, Genetically Modified / drug effects
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Plants, Genetically Modified / genetics*
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Plants, Genetically Modified / radiation effects
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Polyethylene Glycols / pharmacology
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Promoter Regions, Genetic / genetics*
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Ribosome Inactivating Proteins, Type 1 / genetics*
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Salicylic Acid / pharmacology
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Stress, Physiological
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Temperature
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Ultraviolet Rays
Substances
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Plant Proteins
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Ribosome Inactivating Proteins, Type 1
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Polyethylene Glycols
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Abscisic Acid
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Salicylic Acid