Cytomegalovirus blood viral load and hearing loss in young children with congenital infection

Pediatr Infect Dis J. 2009 Jul;28(7):588-92. doi: 10.1097/INF.0b013e3181979a27.


Background: This study was designed to determine whether elevated viral load in infants and young children is associated with congenital cytomegalovirus (CMV)-related hearing loss.

Methods: Blood samples were obtained from 135 children with congenital CMV infection. CMV DNA in the peripheral blood was quantitated with a real-time polymerase chain reaction assay. Viral load measurements were analyzed in 3 different age groups (<2 months, 2-12 months, 12-36 months).

Results: In children with symptomatic and asymptomatic infection, CMV DNA levels were not different between children with hearing deficit and those with normal hearing in all 3 age groups. In children with asymptomatic infection, the positive predictive value of a peripheral blood viral load >3500 genomic equivalents per milliliter (ge/mL) at <2 months and 2 to 12 months of age is 8%, and at 12 to 36 months of age is 11.8%. However, the negative predictive value of a viral load <3500 ge/mL is 94.4% at <2 months of age, and 100% at 2 to 36 months of age.

Conclusions: Peripheral blood viral load is not associated with hearing loss in children with congenital CMV infection. However, a viral load of <3500 ge/mL is associated with a lower risk of hearing loss in children born with asymptomatic congenital infection.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Child, Preschool
  • Cytomegalovirus / isolation & purification*
  • Cytomegalovirus Infections / complications*
  • Cytomegalovirus Infections / virology*
  • DNA, Viral / blood
  • Female
  • Hearing Loss / epidemiology*
  • Hearing Loss / etiology*
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Polymerase Chain Reaction / methods
  • Predictive Value of Tests
  • Risk Factors
  • Viral Load*


  • DNA, Viral