Gastroprotective activity of isopulegol on experimentally induced gastric lesions in mice: investigation of possible mechanisms of action

Naunyn Schmiedebergs Arch Pharmacol. 2009 Sep;380(3):233-45. doi: 10.1007/s00210-009-0429-5. Epub 2009 May 29.


The present study investigated whether isopulegol, a monoterpene present in essential oils of several aromatic plants, would be able to promote some gastroprotective effect and also verified the possible mechanisms involved in this action. For this study, ethanol- and indomethacin-induced gastric ulcer models in mice and histopathological assessment were used. The roles of NO, sulfhydryls (glutathione, GSH), ATP-sensitive K(+) channels (K(ATP) channels), and prostaglandins were also investigated. Isopulegol exhibited a dose-related gastroprotective effect against ethanol-induced lesions, while the pretreatment with glibenclamide and indomethacin [but not with N(G)-nitro-L-arginine methyl ester] were able to reverse this action. The pretreatment with isopulegol also restored GSH levels to normal levels and exhibited dose-related gastroprotective effect against indomethacin-induced ulcer. The results suggested that isopulegol presents significant gastroprotective effects in both ethanol- and indomethacin-induced ulcer models, which appear to be mediated, at least in part, by endogenous prostaglandins, K(ATP) channel opening, and antioxidant properties.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / administration & dosage
  • Antioxidants / pharmacology*
  • Cyclohexane Monoterpenes
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Ethanol / toxicity
  • Glutathione / drug effects
  • Glutathione / metabolism
  • Indomethacin / toxicity
  • KATP Channels / metabolism
  • Male
  • Mice
  • Prostaglandins / metabolism
  • Stomach Ulcer / chemically induced
  • Stomach Ulcer / prevention & control*
  • Terpenes / administration & dosage
  • Terpenes / pharmacology*


  • Antioxidants
  • Cyclohexane Monoterpenes
  • KATP Channels
  • Prostaglandins
  • Terpenes
  • Ethanol
  • isopulegol
  • Glutathione
  • Indomethacin