HOM-C genes, Wnt signaling and axial patterning in the C. elegans posterior ventral epidermis

Dev Biol. 2009 Aug 1;332(1):156-65. doi: 10.1016/j.ydbio.2009.05.567. Epub 2009 May 27.

Abstract

Wnt signaling and HOM-C/Hox genes pattern cell fate along the anterior/posterior axis in many animals. In general, Wnt signaling participates in establishing the anterior/posterior axis, whereas HOM-C genes confer regional identities to cells along the axis. However, recent work in non-bilaterial metazoans suggests that the ancestral patterning system relied on Wnts, with a later co-option of HOM-C genes to replace Wnts in regional patterning. Here we provide direct experimental support for this model from C. elegans, where a regional Wnt patterning system is uncovered in HOM-C gene mutants. Anterior/posterior patterning of P11/P12 cell fate in the C. elegans tail is normally dependent on the HOM-C gene egl-5/Abdominal-B. If the HOM-C gene mab-5/fushi tarazu is also mutant, however, a Wnt signal can promote P12 fate in the absence of egl-5. Furthermore, transcription of egl-5 in the P12.pa cell is influenced by an autoregulatory element that is essential in wild type, but not in mab-5 egl-5 double mutants, identifying regulatory parallels between P12 cell fate specification and egl-5 transcriptional regulation in the P12 lineage. Together, our results identify complex regulatory relationships among signaling pathways and HOM-C genes, and uncover a layering of patterning systems that may reflect their evolutionary history.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Body Patterning* / drug effects
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / drug effects
  • Caenorhabditis elegans / embryology*
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans Proteins / genetics
  • Cell Fusion
  • Cell Lineage / drug effects
  • Conserved Sequence
  • Enhancer Elements, Genetic / genetics
  • Epidermal Cells
  • Epidermal Growth Factor / pharmacology
  • Epidermis / drug effects
  • Epidermis / embryology*
  • Epidermis / metabolism
  • Gene Expression Regulation, Developmental / drug effects
  • Genes, Homeobox*
  • Genes, Reporter
  • Homeostasis / drug effects
  • Models, Biological
  • Molecular Sequence Data
  • Mutation / genetics
  • Signal Transduction* / drug effects
  • Transgenes
  • Wnt Proteins / metabolism*

Substances

  • Caenorhabditis elegans Proteins
  • Wnt Proteins
  • Epidermal Growth Factor