Nuclear localization of ISWI ATPase Smarca5 (Snf2h) in mouse

Front Biosci (Elite Ed). 2009 Jun 1;1:553-9. doi: 10.2741/e53.

Abstract

Nucleosome movement is, at least in part, facilitated by ISWI ATPase Smarca5 (Snf2h). Smarca5 gene inactivation in mouse demonstrated its requirement at blastocyst stage; however its role at later stages is not completely understood. We herein determined nuclear distribution of Smarca5 and histone marks associated with actively transcribed and repressed chromatin structure in embryonic and adult murine tissues and in tumor cells. Confocal microscopy images demonstrate that Smarca5 is localized mainly in euchromatin and to lesser extent also in heterochromatin and nucleoli. Smarca5 heterozygous mice for a null allele display decreased levels of histone H3 modifications and defects in heterochromatin foci supporting role of Smarca5 as a key regulator of global chromatin structure.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / metabolism*
  • Animals
  • Blastocyst / metabolism*
  • Cell Line, Tumor
  • Cell Nucleolus / metabolism*
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Euchromatin / metabolism*
  • Gene Silencing
  • Heterochromatin / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Confocal

Substances

  • Chromosomal Proteins, Non-Histone
  • Euchromatin
  • Heterochromatin
  • Adenosine Triphosphatases
  • Smarca5 protein, mouse