Abstract
Here we describe the protein interaction platform assay, a method for identifying interacting proteins in Saccharomyces cerevisiae. This assay relies on the reovirus scaffolding protein microNS, which forms large focal inclusions in living cells. When a query protein is fused to microNS and potential interaction partners are fused to a fluorescent reporter, interactors can be identified by screening for yeast that display fluorescent foci.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacterial Proteins / genetics
-
Bacterial Proteins / metabolism
-
Mutation
-
Plasmids / genetics
-
Protein Interaction Mapping / methods*
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
-
Saccharomyces cerevisiae / genetics
-
Saccharomyces cerevisiae / metabolism
-
Saccharomyces cerevisiae Proteins / genetics
-
Saccharomyces cerevisiae Proteins / metabolism*
-
Salmonella typhimurium / genetics
-
Salmonella typhimurium / pathogenicity
-
Shigella flexneri / genetics
-
Shigella flexneri / pathogenicity
-
Virulence / genetics
Substances
-
Bacterial Proteins
-
Recombinant Fusion Proteins
-
Saccharomyces cerevisiae Proteins