Classical cadherins control nucleus and centrosome position and cell polarity

J Cell Biol. 2009 Jun 1;185(5):779-86. doi: 10.1083/jcb.200812034.

Abstract

Control of cell polarity is crucial during tissue morphogenesis and renewal, and depends on spatial cues provided by the extracellular environment. Using micropatterned substrates to impose reproducible cell-cell interactions, we show that in the absence of other polarizing cues, cell-cell contacts are the main regulator of nucleus and centrosome positioning, and intracellular polarized organization. In a variety of cell types, including astrocytes, epithelial cells, and endothelial cells, calcium-dependent cadherin-mediated cell-cell interactions induce nucleus and centrosome off-centering toward cell-cell contacts, and promote orientation of the nucleus-centrosome axis toward free cell edges. Nucleus and centrosome off-centering is controlled by N-cadherin through the regulation of cell interactions with the extracellular matrix, whereas the orientation of the nucleus-centrosome axis is determined by the geometry of N-cadherin-mediated contacts. Our results demonstrate that in addition to the specific function of E-cadherin in regulating baso-apical epithelial polarity, classical cadherins control cell polarization in otherwise nonpolarized cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes / metabolism
  • Astrocytes / ultrastructure
  • Cadherins / physiology*
  • Cell Communication
  • Cell Nucleus / metabolism*
  • Cell Nucleus / ultrastructure
  • Cell Polarity*
  • Cells, Cultured
  • Centrosome / metabolism*
  • Centrosome / ultrastructure
  • Intercellular Junctions / metabolism
  • Intercellular Junctions / physiology
  • Models, Biological
  • Rats

Substances

  • Cadherins