Proteomic and tissue array profiling identifies elevated hypoxia-regulated proteins in pancreatic ductal adenocarcinoma

Cancer Invest. 2009 Aug;27(7):747-55. doi: 10.1080/07357900802672746.

Abstract

We identified a group of hypoxia-regulated proteins upregulated in microdissected pancreatic cancer nests compared with normal pancreatic ducts. Immunohistochemical study further validated that pancreatic cancers had significantly higher expression levels of glucose-regulated protein 78, macrophage migration inhibitory factor and annexin A5 than normal pancreas tissues, these protein biomarkers also demonstrated high receiver operating characteristic curves in discriminating pancreatic cancers from normal pancreas. In conclusion, our study indicated a link between pancreatic cancer and hypoxia-regulated proteins. Glucose-regulated protein 78, macrophage migration inhibitory factor and annexin A5 might be promising targets for pancreatic cancer diagnosis and therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Annexin A5 / biosynthesis
  • Annexin A5 / genetics
  • Carcinoma, Pancreatic Ductal / genetics
  • Carcinoma, Pancreatic Ductal / metabolism*
  • Cell Differentiation
  • Cell Hypoxia / genetics*
  • Electrophoresis, Gel, Two-Dimensional
  • Female
  • Gene Expression Profiling*
  • Gene Expression Regulation, Neoplastic*
  • Heat-Shock Proteins / biosynthesis
  • Heat-Shock Proteins / genetics
  • Humans
  • Intramolecular Oxidoreductases / biosynthesis
  • Intramolecular Oxidoreductases / genetics
  • Macrophage Migration-Inhibitory Factors / biosynthesis
  • Macrophage Migration-Inhibitory Factors / genetics
  • Male
  • Middle Aged
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / genetics*
  • Oligonucleotide Array Sequence Analysis
  • Pancreatic Ducts / chemistry
  • Pancreatic Neoplasms / genetics
  • Pancreatic Neoplasms / metabolism*
  • Proteomics*
  • ROC Curve
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Annexin A5
  • Heat-Shock Proteins
  • Macrophage Migration-Inhibitory Factors
  • Neoplasm Proteins
  • Intramolecular Oxidoreductases
  • MIF protein, human
  • molecular chaperone GRP78