UV Melting of G-Quadruplexes

doi:10.1002/0471142700.nc1701s37

Review

. 2009 Jun:Chapter 17:17.1.1-17.1.15.

doi: 10.1002/0471142700.nc1701s37.

UV Melting of G-Quadruplexes

Jean-Louis Mergny¹, Laurent Lacroix¹

Affiliations

PMID: 19488970
DOI: 10.1002/0471142700.nc1701s37

Review

UV Melting of G-Quadruplexes

Jean-Louis Mergny et al. Curr Protoc Nucleic Acid Chem. 2009 Jun.

. 2009 Jun:Chapter 17:17.1.1-17.1.15.

doi: 10.1002/0471142700.nc1701s37.

Authors

Jean-Louis Mergny¹, Laurent Lacroix¹

Affiliation

¹ Laboratoire de Biophysique, Inserm U565, Muséum National d'Histoire Naturelle, Paris, France.

PMID: 19488970
DOI: 10.1002/0471142700.nc1701s37

Abstract

Absorbance versus temperature curves can provide information on the thermal stability of DNA or RNA quadruplexes. Quadruplex denaturation or renaturation can be followed by recording absorbance at 295 nm rather than 260 nm, the wavelength used to monitor duplex denaturation. This unit describes the use of absorbance versus temperature curves to determine melting temperatures (T(m) values) and model-dependent thermodynamic parameters.

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References

Literature Cited

References

1. Albergo, D.D., Marky, L.A., Breslauer, K.J., and Turner, D.H. 1981. Thermodynamics of (dC-dG)3 double helix formation in water and deuterium oxide. Biochemistry 20:1409-1413.
1. Alberti, P., Hoarau, M., Guittat, L., Takasugi, M., Arimondo, P.B., Lacroix, L., Mills, M., Teulade-Fichou, M.P., Vigneron, J.P., Lehn, J.M., Mailliet, P., and Mergny, J.L. 2002. Triplex vs. Quadruplex specific ligands and telomerase inhibition. In Small Molecule DNA and RNA Binders: From Synthesis to Nucleic Acid Complexes (C. Bailly, M. Demeunynck, and D. Wilson, eds.) pp. 315-336. Wiley VCH. Hoboken, N.J.
1. Amrane, S., Saccà, B., Mills, M., Chauhan, M., Klump, H.H., and Mergny, J.L. 2005. Length-dependent energetics of (CTG)n and (CAG)n trinucleotide repeats. Nucleic Acids Res. 33:4065-4077.
1. Bishop, G.R., Ren, J., Polander, B.C., Jeanfreau, B.D., Trent, J.O., and Chaires, J.B. 2007. Energetic basis of molecular recognition in a DNA aptamer. Biophys. Chem. 126:165-175.
1. Cantor, C.R. and Schimmel, P.R. 1980a. Biophysical Chemistry. W.H. Freeman and Co., San Francisco.

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[1] Albergo, D.D., Marky, L.A., Breslauer, K.J., and Turner, D.H. 1981. Thermodynamics of (dC-dG)3 double helix formation in water and deuterium oxide. Biochemistry 20:1409-1413.

[2] Albergo, D.D., Marky, L.A., Breslauer, K.J., and Turner, D.H. 1981. Thermodynamics of (dC-dG)3 double helix formation in water and deuterium oxide. Biochemistry 20:1409-1413.

[3] Alberti, P., Hoarau, M., Guittat, L., Takasugi, M., Arimondo, P.B., Lacroix, L., Mills, M., Teulade-Fichou, M.P., Vigneron, J.P., Lehn, J.M., Mailliet, P., and Mergny, J.L. 2002. Triplex vs. Quadruplex specific ligands and telomerase inhibition. In Small Molecule DNA and RNA Binders: From Synthesis to Nucleic Acid Complexes (C. Bailly, M. Demeunynck, and D. Wilson, eds.) pp. 315-336. Wiley VCH. Hoboken, N.J.

[4] Alberti, P., Hoarau, M., Guittat, L., Takasugi, M., Arimondo, P.B., Lacroix, L., Mills, M., Teulade-Fichou, M.P., Vigneron, J.P., Lehn, J.M., Mailliet, P., and Mergny, J.L. 2002. Triplex vs. Quadruplex specific ligands and telomerase inhibition. In Small Molecule DNA and RNA Binders: From Synthesis to Nucleic Acid Complexes (C. Bailly, M. Demeunynck, and D. Wilson, eds.) pp. 315-336. Wiley VCH. Hoboken, N.J.

[5] Amrane, S., Saccà, B., Mills, M., Chauhan, M., Klump, H.H., and Mergny, J.L. 2005. Length-dependent energetics of (CTG)n and (CAG)n trinucleotide repeats. Nucleic Acids Res. 33:4065-4077.

[6] Amrane, S., Saccà, B., Mills, M., Chauhan, M., Klump, H.H., and Mergny, J.L. 2005. Length-dependent energetics of (CTG)n and (CAG)n trinucleotide repeats. Nucleic Acids Res. 33:4065-4077.

[7] Bishop, G.R., Ren, J., Polander, B.C., Jeanfreau, B.D., Trent, J.O., and Chaires, J.B. 2007. Energetic basis of molecular recognition in a DNA aptamer. Biophys. Chem. 126:165-175.

[8] Bishop, G.R., Ren, J., Polander, B.C., Jeanfreau, B.D., Trent, J.O., and Chaires, J.B. 2007. Energetic basis of molecular recognition in a DNA aptamer. Biophys. Chem. 126:165-175.

[9] Cantor, C.R. and Schimmel, P.R. 1980a. Biophysical Chemistry. W.H. Freeman and Co., San Francisco.

[10] Cantor, C.R. and Schimmel, P.R. 1980a. Biophysical Chemistry. W.H. Freeman and Co., San Francisco.

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UV Melting of G-Quadruplexes

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UV Melting of G-Quadruplexes

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