Super-resolution fluorescence microscopy

Annu Rev Biochem. 2009;78:993-1016. doi: 10.1146/annurev.biochem.77.061906.092014.

Abstract

Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional fluorescence microscopy. New advances in these techniques now give them the ability to image three-dimensional (3D) structures, measure interactions by multicolor colocalization, and record dynamic processes in living cells at the nanometer scale. It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cytological Techniques
  • Humans
  • Imaging, Three-Dimensional
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods*