Reduced ability of hemagglutinin of the CAM-70 measles virus vaccine strain to use receptors CD46 and SLAM

Vaccine. 2009 Jun 12;27(29):3838-48. doi: 10.1016/j.vaccine.2009.04.010. Epub 2009 Apr 23.


The CAM-70 measles virus (MV) vaccine strain is currently used for vaccination against measles. We examined the fusion-inducing ability of the CAM-70 hemagglutinin (H) protein and found that it was impaired in both CD46- and signaling lymphocyte activation molecule (SLAM)-expressing cells. We also generated recombinant MVs possessing H genes derived from the CAM-70 strain. The CAM-70 H protein impaired viral growth in both CD46- and SLAM-expressing cells. In peripheral blood lymphocytes (PBL) and monocyte-derived dendritic cells (Mo-DC), the CAM-70 strain did not grow efficiently. Infection with recombinant MVs revealed that impaired growth of the CAM-70 strain was attributed to the H gene only partly in PBL and largely in Mo-DC. Thus, impaired fusion-inducing ability of the H protein may be one of the underlying molecular mechanisms resulting in the attenuation of the CAM-70 strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution / genetics
  • Animals
  • Antigens, CD / metabolism*
  • Cells, Cultured
  • Chlorocebus aethiops
  • Dendritic Cells / virology
  • HeLa Cells
  • Hemagglutinins, Viral / genetics
  • Hemagglutinins, Viral / metabolism*
  • Humans
  • Lymphocytes / virology
  • Measles virus / genetics
  • Measles virus / physiology*
  • Membrane Cofactor Protein / metabolism*
  • Molecular Sequence Data
  • Protein Binding
  • Receptors, Cell Surface / metabolism*
  • Receptors, Virus / metabolism*
  • Sequence Analysis, DNA
  • Signaling Lymphocytic Activation Molecule Family Member 1
  • Vero Cells
  • Virus Attachment*


  • Antigens, CD
  • CD46 protein, human
  • Hemagglutinins, Viral
  • Membrane Cofactor Protein
  • Receptors, Cell Surface
  • Receptors, Virus
  • hemagglutinin protein G, measles virus
  • Signaling Lymphocytic Activation Molecule Family Member 1

Associated data

  • GENBANK/AB467283