Assessing human immunodeficiency virus type 1 tropism: Comparison of assays using replication-competent virus versus plasma-derived pseudotyped virions

J Clin Microbiol. 2009 Aug;47(8):2604-6. doi: 10.1128/JCM.00632-09. Epub 2009 Jun 3.


Detection of CXCR4-using human immunodeficiency virus by the Trofile assay was compared to that by assays using virus isolates or replication-competent recombinants. Concordance with the Trofile assay was good, but assays using replicating viruses did not increase substantially the ability to detect the presence of CXCR4-using virus.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • HIV-1 / genetics
  • HIV-1 / physiology*
  • Humans
  • Plasma / virology
  • Receptors, Virus / analysis*
  • Sensitivity and Specificity
  • Virology / methods*
  • Virus Internalization*
  • Virus Replication*


  • Receptors, Virus