Bioreduction of alpha-chloroacetophenone by whole cells of marine fungi

Biotechnol Lett. 2009 Oct;31(10):1559-63. doi: 10.1007/s10529-009-0037-y. Epub 2009 Jun 3.

Abstract

The asymmetric reduction of 2-chloro-1-phenylethanone (1) by seven strains of marine fungi was evaluated and afforded (S)-(-)-2-chloro-1-phenylethanol with, in the best case, an enantiomeric excess of 50% and an isolated yield of 60%. The ability of marine fungi to catalyse the reduction was directly dependent on growth in artificial sea water-based medium containing a high concentration of Cl(-) (1.2 M). When fungi were grown in the absence of artificial sea water, no reduction of 1 by whole cells was observed. The biocatalytic reduction of 1 was more efficient at neutral rather than acidic pH values and in the absence of glucose as co-substrate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotransformation
  • Culture Media / chemistry
  • Fungi / isolation & purification
  • Fungi / metabolism*
  • Oxidation-Reduction
  • Seawater / microbiology*
  • omega-Chloroacetophenone / metabolism*

Substances

  • Culture Media
  • omega-Chloroacetophenone