Parallax: high accuracy three-dimensional single molecule tracking using split images

Nano Lett. 2009 Jul;9(7):2676-82. doi: 10.1021/nl901129j.


Three-dimensional (3D) tracking can provide valuable biological insights that are missing in conventional microscopy. Here we developed a single molecule 3D tracking microscopy technique, named Parallax, with high localization precision and temporal resolution. We demonstrated its capabilities by studying the 3D trafficking of glucose-transporter-4 containing vesicles in living adipocytes as well as the walking path of single myosin VI molecules along actin filaments.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adipocytes / chemistry
  • Animals
  • Glucose Transporter Type 4 / chemistry
  • Image Interpretation, Computer-Assisted / methods
  • Imaging, Three-Dimensional / methods*


  • Glucose Transporter Type 4