Extra-cellular signal regulated kinase (ERK) signal transduction is an important signaling pathway, involved in a variety of processes in cell and is closely related with the occurrence of human cancers. By the application of dynamic method, the ERK signal pathway activated by epidermal growth factor receptor (EGFR) was simulated and analyzed in this work. The influence of the mutation of adaptor protein on the expression of phosphorylated ERK signal was analyzed, and the complex coupling of the protein kinases and their kinetic function were analyzed. The obtained results are helpful to understanding the mechanism of tumor development and to finding out new target molecules in the ERK signaling system.