Abstract
We investigated anti-inflammatory effects of two coumarins, columbianetin (A) and libanoridin (B), isolated from Corydalis heterocarpa in lipopolysaccharide (LPS)-stimulated HT-29 human colon carcinoma cells. Treatment with compound B inhibited the protein expression levels of inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) in a dose-dependent manner in LPS-stimulated HT-29 cells, but compound A did not. Also, compound B had a higher inhibitory effect on production of cytokines such as IL-1 beta and TNF-alpha in LPS-stimulated HT-29 human colon carcinoma cells than those of compound A. Furthermore, we confirmed that LPS-induced transcription activity of NF-kappaB was inhibited by compound B. As a result of this study, compound B can be considered as a potential anti-inflammatory agent.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification*
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology
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Blotting, Western
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Cell Survival / drug effects
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Coloring Agents
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Corydalis / chemistry*
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Coumarins / isolation & purification*
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Coumarins / pharmacology*
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Dose-Response Relationship, Drug
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Enzyme-Linked Immunosorbent Assay
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Furocoumarins / isolation & purification*
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Furocoumarins / pharmacology*
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HT29 Cells
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Humans
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Inflammation Mediators / analysis
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Lipopolysaccharides / pharmacology
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Magnetic Resonance Spectroscopy
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Spectrometry, Mass, Electrospray Ionization
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Spectrophotometry, Ultraviolet
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Tetrazolium Salts
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Thiazoles
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Antineoplastic Agents, Phytogenic
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Coloring Agents
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Coumarins
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Furocoumarins
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Inflammation Mediators
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Lipopolysaccharides
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Tetrazolium Salts
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Thiazoles
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libanoridin
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columbianetin
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thiazolyl blue