Nitric oxide-releasing S-nitrosothiol-modified xerogels

Biomaterials. 2009 Sep;30(27):4494-502. doi: 10.1016/j.biomaterials.2009.05.006. Epub 2009 Jun 6.

Abstract

The synthesis, material characterization, and in vitro biocompatibility of S-nitrosothiol (RSNO)-modified xerogels are described. Thiol-functionalized xerogel films were formed by hydrolysis and co-condensation of 3-mercaptopropyltrimethoxysilane (MPTMS) and methyltrimethoxysilane (MTMOS) sol-gel precursors at varying concentrations. Subsequent thiol nitrosation via acidified nitrite produced RSNO-modified xerogels capable of generating nitric oxide (NO) for up to 2 weeks under physiological conditions. Xerogels also exhibited NO generation upon irradiation with broad-spectrum light or exposure to copper, with NO fluxes proportional to wattage and concentration, respectively. Xerogels were capable of storing up to approximately 1.31 micromol NO mg(-1), and displayed negligible fragmentation over a 2-week period. Platelet and bacterial adhesion to nitrosated films was reduced compared to non-nitrosated controls, confirming the antithrombotic and antibacterial properties of the NO-releasing materials. Fibroblast cell viability was maintained on the xerogel surfaces illustrating the promise of RSNO-modified xerogels as biomedical device coatings.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Antithrombins / pharmacology
  • Bacterial Adhesion / drug effects
  • Blood Platelets / drug effects
  • Blood Platelets / ultrastructure
  • Cell Survival / drug effects
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Gels / metabolism*
  • Mice
  • Microbial Sensitivity Tests
  • Nitric Oxide / metabolism*
  • Platelet Activation / drug effects
  • Pseudomonas aeruginosa / drug effects
  • S-Nitrosothiols / metabolism*
  • Silanes / metabolism
  • Sus scrofa

Substances

  • Anti-Bacterial Agents
  • Antithrombins
  • Gels
  • S-Nitrosothiols
  • Silanes
  • methyltrimethoxysilane
  • Nitric Oxide
  • (3-mercaptopropyl)trimethoxysilane