Response to ODN-CpG by B Cells from patients with systemic lupus erythematosus correlates with disease activity

Lupus. 2009 Jul;18(8):718-26. doi: 10.1177/0961203309103098.

Abstract

Different immunological alterations may condition systemic lupus erythematosus (SLE) activity. However, it is not known whether alterations in the phenotype of circulating antigen-presenting cells (APCs) and in the response to CpG oligodeoxynucleotides (ODN-CpG) correlate with disease activity. APC expression of HLA-DR, costimulatory molecules, and TLR9 expression was determined in patients with SLE, other autoimmune diseases, and healthy controls. Monocyte and B cell response to synthetic ODN-CpG sequences was also evaluated. Monocytes from patients with moderate SLE activity had higher expression of CD40 and CD86. Decreased numbers of CD19+CD80+ and BDCA-3+CD40+ cells were found in patients with severe SLE activity. In patients with moderate SLE activity, non-adherent and enriched B cell response to ODN-CpG was similar to healthy controls. Adherent and enriched B cells from patients with severe SLE activity did not increase costimulatory molecule expression or cytokine production after ODN-CpG stimulation. APCs from patients with SLE, regardless of disease activity, displayed higher percentage of TLR9+ cells, as well as increased expression of TLR9, compared to healthy controls. Results suggest that the B cell response to ODN-CpG correlates with the SLE activity, independently of TLR9 expression, indicating that alterations in B cell response in severe activity SLE may be caused by events down-stream to TLR9.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic*
  • Adolescent
  • Adult
  • Aged
  • Antigens, CD / immunology
  • Antigens, Surface / immunology
  • Dendritic Cells / immunology
  • Female
  • HLA-DR Antigens / immunology
  • Humans
  • Lupus Erythematosus, Systemic* / immunology
  • Lupus Erythematosus, Systemic* / physiopathology
  • Male
  • Middle Aged
  • Monocytes / immunology
  • Oligodeoxyribonucleotides / immunology*
  • Toll-Like Receptor 9 / immunology
  • Young Adult

Substances

  • Adjuvants, Immunologic
  • Antigens, CD
  • Antigens, Surface
  • CPG-oligonucleotide
  • HLA-DR Antigens
  • Oligodeoxyribonucleotides
  • TLR9 protein, human
  • Toll-Like Receptor 9
  • blood dendritic cell antigen 3, human