Identification of non-tuberculous mycobacteria: utility of the GenoType Mycobacterium CM/AS assay compared with HPLC and 16S rRNA gene sequencing

J Med Microbiol. 2009 Jul;58(Pt 7):900-904. doi: 10.1099/jmm.0.007484-0. Epub 2009 Jun 5.


Non-tuberculous mycobacteria (NTM) causing clinical disease have become increasingly common and more diverse. A new reverse line probe assay, GenoType Mycobacterium CM/AS (Hain Lifescience), was evaluated for identification of a broad range of NTM. It was compared with phenotypic (HPLC) and molecular (DNA probes, in-house real-time multiplex species-specific PCR, 16S rRNA gene PCR and sequencing) identification techniques, which together provided the reference 'gold standard'. A total of 131 clinical isolates belonging to 31 Mycobacterium species and 19 controls, including 5 non-Mycobacterium species, was used. Concordant results between the GenoType Mycobacterium assay and the reference identification were obtained in 119/131 clinical isolates (90.8 %). Identification of Mycobacterium abscessus and Mycobacterium lentiflavum by the assay was problematic. The GenoType Mycobacterium assay enables rapid identification of a broad range of potentially clinically significant Mycobacterium species, but some species require further testing to differentiate or confirm ambiguous results.

Publication types

  • Comparative Study

MeSH terms

  • Bacteriological Techniques*
  • Chromatography, High Pressure Liquid / methods*
  • Genotype
  • Mycobacterium / classification*
  • Mycobacterium / isolation & purification*
  • RNA, Bacterial / genetics*
  • RNA, Ribosomal, 16S / genetics*
  • Sensitivity and Specificity


  • RNA, Bacterial
  • RNA, Ribosomal, 16S